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Title: Agrobacterium  
Author: World Heritage Encyclopedia
Language: English
Subject: Plant genetics, Genetically modified crops, Genetically modified virus, Genetically modified organism, Genetically modified wheat
Collection: Biotechnology, Rhizobiaceae
Publisher: World Heritage Encyclopedia


Agrobacterium is a genus of Gram-negative bacteria established by H. J. Conn that uses horizontal gene transfer to cause tumors in plants. Agrobacterium tumefaciens is the most commonly studied species in this genus. Agrobacterium is well known for its ability to transfer DNA between itself and plants, and for this reason it has become an important tool for genetic engineering.

The Agrobacterium genus is quite heterogeneous. Recent taxonomic studies have reclassified all of the Agrobacterium species into new genera, such as Ahrensia, Pseudorhodobacter, Ruegeria, and Stappia,[1][2] but most species have been controversially reclassified as Rhizobium species.[3][4][5]


  • Plant pathogen 1
  • Agrobacterium in humans 2
  • Uses in biotechnology 3
  • Genomics 4
  • See also 5
  • References 6
  • External links 7

Plant pathogen

The large growths on these roots are galls induced by Agrobacterium sp.

A. tumefaciens causes crown-gall disease in plants. The disease is characterised by a tumour-like growth or gall on the infected plant, often at the junction between the root and the shoot. Tumors are incited by the conjugative transfer of a DNA segment (T-DNA) from the bacterial tumour-inducing (Ti) plasmid. The closely related species, A. rhizogenes, induces root tumors, and carries the distinct Ri (root-inducing) plasmid. Although the taxonomy of Agrobacterium is currently under revision it can be generalised that 3 biovars exist within the genus, A. tumefaciens, A. rhizogenes, and A. vitis. Strains within A. tumefaciens and A. rhizogenes are known to be able to harbour either a Ti or Ri-plasmid, whilst strains of A. vitis, generally restricted to grapevines, can harbour a Ti-plasmid. Non-Agrobacterium strains have been isolated from environmental samples which harbour a Ri-plasmid whilst laboratory studies have shown that non-Agrobacterium strains can also harbour a Ti-plasmid. Many environmental strains of Agrobacterium possess neither a Ti nor Ri-plasmid. These strains are of course avirulent.

The plasmid T-DNA is integrated semi-randomly into the selective advantage.[7] By altering the hormone balance in the plant cell, the division of those cells cannot be controlled by the plant, and tumors form. The ratio of auxin to cytokinin produced by the tumor genes determines the morphology of the tumor (root-like, disorganized or shoot-like).

Agrobacterium in humans

Although generally seen as an infection in plants, Agrobacterium can be responsible for opportunistic infections in humans with weakened immune systems,[8][9] but has not been shown to be a primary pathogen in otherwise healthy individuals. One of the earliest associations of human disease caused by Agrobacterium radiobacter was reported by Dr. J. R. Cain in Scotland (1988).[10] A later study suggested that Agrobacterium attaches to and genetically transforms several types of human cells by integrating its T-DNA into the human cell genome. The study was conducted using cultured human tissue and did not draw any conclusions regarding related biological activity in nature.[11]

Uses in biotechnology

The ability of Agrobacterium to transfer genes to plants and fungi is used in biotechnology, in particular, genetic engineering for plant improvement. A modified Ti or Ri plasmid can be used. The plasmid is 'disarmed' by deletion of the tumor inducing genes; the only essential parts of the T-DNA are its two small (25 base pair) border repeats, at least one of which is needed for plant transformation. Marc Van Montagu and Jozef Schell at the University of Ghent (Belgium) discovered the gene transfer mechanism between Agrobacterium and plants, which resulted in the development of methods to alter Agrobacterium into an efficient delivery system for gene engineering in plants.[12][13] A team of researchers led by Dr Mary-Dell Chilton were the first to demonstrate that the virulence genes could be removed without adversely affecting the ability of Agrobacterium to insert its own DNA into the plant genome (1983).

The genes to be introduced into the plant are cloned into a plant transformation vector that contains the T-DNA region of the disarmed plasmid, together with a selectable marker (such as antibiotic resistance) to enable selection for plants that have been successfully transformed. Plants are grown on media containing antibiotic following transformation, and those that do not have the T-DNA integrated into their genome will die. An alternative method is agroinfiltration.

Plant (S. chacoense) transformed using Agrobacterium. Transformed cells start forming calluses on the side of the leaf pieces

Transformation with Agrobacterium can be achieved in two ways. Protoplasts or alternatively leaf-discs can be incubated with the Agrobacterium and whole plants regenerated using plant tissue culture. A common transformation protocol for Arabidopsis is the floral-dip method: the flowers are dipped in an Agrobacterium culture, and the bacterium transforms the germline cells that make the female gametes. The seeds can then be screened for antibiotic resistance (or another marker of interest), and plants that have not integrated the plasmid DNA will die.

Agrobacterium does not infect all plant species, but there are several other effective techniques for plant transformation including the gene gun.

Agrobacterium is listed as being the original source of genetic material that was transferred to these USA GMOs:[14]


The sequencing of the genes and systems involved in pathogenesis, biological control and symbiosis. One important finding is the possibility that chromosomes are evolving from plasmids in many of these bacteria. Another discovery is that the diverse chromosomal structures in this group appear to be capable of supporting both symbiotic and pathogenic lifestyles. The availability of the genome sequences of Agrobacterium species will continue to increase, resulting in substantial insights into the function and evolutionary history of this group of plant-associated microbes.[15]

See also


  1. ^ Uchino, Yoshihito; Yokota, Akira; Sugiyama, Junta (1997). "Phylogenetic position of the marine subdivision of Agrobacterium species based on 16S rRNA sequence analysis". The Journal of General and Applied Microbiology 43 (4): 243–247.  
  2. ^ Uchino, Yoshihito; Hirata, Aiko; Yokota, Akira; Sugiyama, Junta (1998). "Reclassification of marine Agrobacterium species: Proposals of Stappia stellulata gen. nov., comb. Nov., Stappia aggregata sp. nov., nom. Rev., Ruegeria atlantica gen. nov., comb. Nov., Ruegeria gelatinovora comb. Nov., Ruegeria algicola comb. Nov., and Ahrensia kieliense gen. nov., sp. nov., nom. Rev". The Journal of General and Applied Microbiology 44 (3): 201–210.  
  3. ^ Young, J. M.; Kuykendall, L. D.; Martínez-Romero, E; Kerr, A; Sawada, H (2001). "A revision of Rhizobium Frank 1889, with an emended description of the genus, and the inclusion of all species of Agrobacterium Conn 1942 and Allorhizobium undicola de Lajudie et al. 1998 as new combinations: Rhizobium radiobacter, R. Rhizogenes, R. Rubi, R. Undicola and R. Vitis". International journal of systematic and evolutionary microbiology 51 (Pt 1): 89–103.  
  4. ^ Farrand, S. K.; Van Berkum, P. B.; Oger, P (2003). "Agrobacterium is a definable genus of the family Rhizobiaceae". International Journal of Systematic and Evolutionary Microbiology 53 (5): 1681–7.  
  5. ^ Young, J. M.; Kuykendall, L. D.; Martínez-Romero, E; Kerr, A; Sawada, H (2003). "Classification and nomenclature of Agrobacterium and Rhizobium - a reply to Farrand et al. (2003)". International Journal of Systematic and Evolutionary Microbiology 53 (5): 1689–95.  
  6. ^ Francis, Kirk E.; Spiker, Steven (2004). "Identification of Arabidopsis thaliana transformants without selection reveals a high occurrence of silenced T-DNA integrations". The Plant Journal 41 (3): 464–77.  
  7. ^ Pitzschke, Andrea; Hirt, Heribert (2010). "New insights into an old story: Agrobacterium-induced tumour formation in plants by plant transformation". The EMBO Journal 29 (6): 1021–32.  
  8. ^ Hulse, M.; Johnson, S.; Ferrieri, P. (1993). "Agrobacterium Infections in Humans: Experience at One Hospital and Review". Clinical Infectious Diseases 16 (1): 112–7.  
  9. ^ Dunne Jr, W. M.; Tillman, J; Murray, J. C. (1993). "Recovery of a strain of Agrobacterium radiobacter with a mucoid phenotype from an immunocompromised child with bacteremia". Journal of clinical microbiology 31 (9): 2541–3.  
  10. ^ Cain, John Raymond (1988). "A case of septicaemia caused by Agrobacterium radiobacter". Journal of Infection 16 (2): 205–6.  
  11. ^ Kunik, T.; Tzfira, T; Kapulnik, Y; Gafni, Y; Dingwall, C; Citovsky, V (2001). "Agrobacterium"Genetic transformation of HeLa cells by . Proceedings of the National Academy of Sciences 98 (4): 1871–6.  
  12. ^ Schell, J.; Van Montagu, M. (1977). "The Ti-Plasmid of Agrobacterium Tumefaciens, A Natural Vector for the Introduction of NIF Genes in Plants?". In Hollaender, Alexander; Burris, R. H.; Day, P. R.; Hardy, R. W. F.; Helinski, D. R.; Lamborg, M. R.; Owens, L.; Valentine, R. C. Genetic Engineering for Nitrogen Fixation. Basic Life Sciences 9. pp. 159–79.  
  13. ^ Joos, H; Timmerman, B; Montagu, M. V.; Schell, J (1983). "Genetic analysis of transfer and stabilization of Agrobacterium DNA in plant cells". The EMBO journal 2 (12): 2151–60.  
  14. ^ The FDA List of Completed Consultations on Bioengineered Foods
  15. ^ Setubal, Joao C.; WOod, Derek; Burr, Thomas; Farrand, Stephen K.; Goldman, Barry S.; Goodner, Brad; Otten, Leon; Slater, Steven (2009). : Insights into its Pathogenicity, Biocontrol, and Evolution"Agrobacterium"The Genomics of . In Jackson, Robert W. Plant Pathogenic Bacteria: Genomics and Molecular Biology. Caister Academic Press. pp. 91–112.  

External links

  • Kyndt, Tina; Quispe, Dora; Zhai, Hong; Jarret, Robert; Ghislain, Marc; Liu, Qingchang; Gheysen, Godelieve; Kreuze, Jan F. (2015). "The genome of cultivated sweet potato contains Agrobacterium T-DNAs with expressed genes: An example of a naturally transgenic food crop". Proceedings of the National Academy of Sciences: 201419685.  
  • namesRhizobium species, and new AgrobacteriumCurrent taxonomy of
  • Agrobacteria is used as gene ferry - Plant transformation with Agrobacterium]
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