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Title: Grlf1  
Author: World Heritage Encyclopedia
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Subject: Transcription factors, NeuroD, EMX homeogene, Engrailed (gene), Nur (biology)
Collection: Transcription Factors
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Rho GTPase activating protein 35

Rendering based on PDB .
Available structures
PDB Ortholog search: PDBe, RCSB
Symbols  ; GRF-1; GRLF1; P190-A; P190A; p190ARhoGAP; p190RhoGAP
External IDs GeneCards:
RNA expression pattern
Species Human Mouse
RefSeq (mRNA)
RefSeq (protein)
Location (UCSC)
PubMed search

Glucocorticoid receptor DNA-binding factor 1 is a protein that in humans is encoded by the GRLF1 gene.[1][2] The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids.[2]


  1. ^ LeClerc S, Palaniswami R, Xie BX, Govindan MV (Oct 1991). "Molecular cloning and characterization of a factor that binds the human glucocorticoid receptor gene and represses its expression". J Biol Chem 266 (26): 17333–40.  
  2. ^ a b "Entrez Gene: GRLF1 glucocorticoid receptor DNA binding factor 1". 

Further reading

  • Nakajima D, Okazaki N, Yamakawa H, et al. (2003). "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones". DNA Res. 9 (3): 99–106.  
  • Warriar N, Pagé N, Govindan MV (1996). "Expression of human glucocorticoid receptor gene and interaction of nuclear proteins with the transcriptional control element". J. Biol. Chem. 271 (31): 18662–71.  
  • Tikoo A, Czekay S, Viars C, et al. (2001). "p190-A, a human tumor suppressor gene, maps to the chromosomal region 19q13.3 that is reportedly deleted in some gliomas". Gene 257 (1): 23–31.  
  • Nagase T, Kikuno R, Hattori A, et al. (2001). "Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 7 (6): 347–55.  
  • Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903.  
  • Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5.  
  • Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5.  
  • Rush J, Moritz A, Lee KA, et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94–101.  
  • Barberis D, Casazza A, Sordella R, et al. (2006). "p190 Rho-GTPase activating protein associates with plexins and it is required for semaphorin signalling". J. Cell. Sci. 118 (Pt 20): 4689–700.  
  • Holinstat M, Knezevic N, Broman M, et al. (2006). "Suppression of RhoA activity by focal adhesion kinase-induced activation of p190RhoGAP: role in regulation of endothelial permeability". J. Biol. Chem. 281 (4): 2296–305.  
  • Oh JH, Yang JO, Hahn Y, et al. (2006). "Transcriptome analysis of human gastric cancer". Mamm. Genome 16 (12): 942–54.  
  • Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell 127 (3): 635–48.  
  • Mammoto T, Parikh SM, Mammoto A, et al. (2007). "Angiopoietin-1 requires p190 RhoGAP to protect against vascular leakage in vivo". J. Biol. Chem. 282 (33): 23910–8.  

External links

  • GRLF1 protein, human at the US National Library of Medicine Medical Subject Headings (MeSH)
  • GRLF1 Info with links in the Cell Migration Gateway

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

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