World Library  
Flag as Inappropriate
Email this Article

Neurogenetics

Article Id: WHEBN0018143331
Reproduction Date:

Title: Neurogenetics  
Author: World Heritage Encyclopedia
Language: English
Subject: Neuroscience, Neurogenetics (journal), Wim Crusio, Journal of Neurogenetics, Seymour Benzer
Collection: Genetics, Neuroscience
Publisher: World Heritage Encyclopedia
Publication
Date:
 

Neurogenetics

Human karyogram

Neurogenetics studies the role of traits. Mutations in this genetic sequence can have a wide range of effects on the quality of life of the individual. Neurological diseases, behavior and personality are all aspects of man studied in the context of neurogenetics. The field of neurogenetics emerged in the mid to late 1900s with advances closely following advancements made in available technology. Currently neurogenetics is the center of much research utilizing the cutting edge of research techniques.

Contents

  • History 1
  • Neurological disorders 2
  • Gene sequencing 3
  • Methods of research 4
    • Statistical Analysis 4.1
    • Recombinant DNA 4.2
    • Animal research 4.3
    • Human research 4.4
  • Behavioral neurogenetics 5
    • Cross-species gene conservation 5.1
    • Impulse control 5.2
    • Higher cognitive function 5.3
    • Aggression 5.4
    • Alcohol dependency 5.5
  • Development 6
  • Current research 7
  • See also 8
  • References 9

History

The field of neurogenetics emerged from advances made in molecular biology, genetics and a desire to understand the link between genes, behavior, the brain, and neurological disorders and diseases. The field started to expand in the 1960s through the research of Seymour Benzer, considered by some to be the father of neurogenetics.[1]
Seymour Benzer in his office at Caltech in 1974 with a big model of Drosophila
His pioneering work with Drosophila helped to elucidate the link between circadian rhythms and genes, which led to further investigations into other behavior traits. He also started conducting research in neurodegeneration in fruit flies in an attempt to discover ways to suppress neurological diseases in humans. Many of the techniques he used and conclusions he drew would drive the field forward.[2]

Early analysis relied on statistical interpretation through processes such as LOD (logarithm of odds) scores of pedigrees and other observational methods such as affected sib-pairs, which looks at phenotype and IBD (identity by descent) configuration. Many of the disorders studied early on including Alzheimer’s, Huntington's and amyotrophic lateral sclerosis (ALS) are still at the center of much research to this day.[3] By the late 1980s new advances in genetics such as recombinant DNA technology and reverse genetics allowed for the broader use of DNA polymorphisms to test for linkage between DNA and gene defects. This process is referred to sometimes as linkage analysis.[4][5] By the 1990s ever advancing technology had made genetic analysis more feasible and available. This decade saw a marked increase in identifying the specific role genes played in relation to neurological disorders. Advancements were made in but not limited to: Fragile X syndrome, Alzheimer’s, Parkinson’s, epilepsy and ALS.[6]

Neurological disorders

While the genetic basis of simple diseases and disorders has been accurately pinpointed, the genetics behind more complex, neurological disorders is still a source of ongoing research. New developments such as the genome wide association studies (GWAS) have brought vast new resources within grasp. With this new information genetic variability within the human population and possibly linked diseases can be more readily discerned.[7] Neurodegenerative diseases are a more common subset of neurological disorders, with examples being Alzheimer's disease and Parkinson's disease. Currently no viable treatments exist that actually reverse the progression of neurodegenerative diseases; however, neurogenetics is emerging as one field that might yield a causative connection. The discovery of linkages could then lead to therapeutic drugs, which could reverse brain degeneration.[8]

Gene sequencing

One of the most noticeable results of further research into neurogenetics is a greater knowledge of gene loci that show linkage to neurological diseases. The table below represents a sampling of specific gene locations identified to play a role in selected neurological diseases based on prevalence in the United States.[9][10][11][12]
Gene Loci Neurological Disease
APOE ε4, PICALM[10] Alzheimer's Disease
DR15, DQ6[11] Multiple Sclerosis
LRRK2, PARK2, PARK7[9] Parkinson's Disease
HTT[12] Huntington's Disease

Methods of research

Statistical Analysis

Logarithm of odds (LOD) is a statistical technique used to estimate the probability of gene linkage between traits. LOD is often used in conjunction with pedigrees, maps of a family’s genetic make-up, in order to yield more accurate estimations. A key benefit of this technique is its ability to give reliable results in both large and small sample sizes, which is a marked advantage in laboratory research.[13][14]

  1. ^
  2. ^
  3. ^
  4. ^
  5. ^
  6. ^
  7. ^
  8. ^
  9. ^ a b
  10. ^ a b
  11. ^ a b
  12. ^ a b
  13. ^ N E Morton (1996). Logarithm of odds (lods) for linkage on complex inheritance
  14. ^ Helms, Ted (2000) Logarithm of Odds in Advanced Genetics.
  15. ^ R. W. Williams (1998) Neuroscience Meets Quantitative Genetics: Using Morphometric Data to Map Genes that Modulate CNS Architecture.
  16. ^
  17. ^ Kuure-Kinsey, Matthew; McCooey, Beth (2006). The Basics of Recombinant DNA.
  18. ^ Ambrose, Victor (2011). Reverse Genetics.
  19. ^ a b Pfeiffer, Barret D, et al. (2008) DrosophilaTools for neuroanatomy and neurogenetics in .
  20. ^ a b Rand, James B, Duerr, Janet S, Frisby, Dennis L (2000) C. elegansNeurogenetics of vesicular transporters in .
  21. ^ Burgess, Harold A, Granato, Michael (2008) The neurogenetic frontier – lessons from misbehaving zebrafish.
  22. ^ McGraw, Lisa A, Young, Larry J (2009) The prairie vole: and emerging model organism for understanding the social brain.
  23. ^ Neurogenetics and Behavior Center. Johns Hopkins U, 2011. Web. 29 Oct. 2011.
  24. ^ Fu, Ying-Hui, and Louis Ptacek, dirs. "Research Projects." Fu and Ptacek's Laboratories of Neurogenetics. U of California, San Francisco, n.d. Web. 29 Oct. 2011..
  25. ^ "Testing Services." Medical Neurogenetics. N.p., 2010. Web. 29 Oct. 2011..
  26. ^ a b c d e
  27. ^ a b c
  28. ^ a b c
  29. ^ a b
  30. ^ a b
  31. ^
  32. ^ Waelti P, Dickinson A, Schultz W Dopamine responses comply with basic assumptions of formal learning theory. Nature. 2001 Jul 5;412(6842):43-8.
  33. ^
  34. ^ a b
  35. ^
  36. ^
  37. ^ Laura Sanders (2011). Brain gene activity changes through life
  38. ^
  39. ^ "This Week In the Journal." The Journal of Neuroscience.

References

See also

Neurogenetics is a field that is rapidly expanding and growing. The current areas of research are very diverse in their focuses. One area deals with molecular processes and the function of certain proteins, often in conjunction with cell signaling and neurotransmitter release, cell development and repair, or neuronal plasticity. Behavioral and cognitive areas of research continue to expand in an effort to pinpoint contributing genetic factors. As a result of the expanding neorogenetics field a better understanding of specific neurological disorders and phenotypes has arisen with direct correlation to genetic mutations. With severe disorders such as epilepsy, brain malformations, or mental retardation a single gene or causative condition has been identified 60% of the time; however, the milder the intellectual handicap the lower chance a specific genetic cause has been pinpointed. Autism for example is only linked to a specific, mutated gene about 15-20% of the time while the mildest forms of mental handicaps are only being accounted for genetically less than 5% of the time. Research in neurogenetics has yielded some promising results, though, in that mutations at specific gene loci have been linked to harmful phenotypes and their resulting disorders. For instance a frameshift mutation or a missense mutation at the DCX gene location causes a neuronal migration defect also known as lissencephaly. Another example is the ROBO3 gene where a mutation alters axon length negatively impacting neuronal connections. Horizontal gaze palsy with progressive scoliosis (HGPPS) accompanies a mutation here.[38] These are just a few examples of what current research in the field of neurogenetics has achieved.[39]

Current research

Some recent research has shown that the level of gene expression changes drastically in the brain at different periods throughout the life cycle. For example, during prenatal development the amount of mRNA in the brain (an indicator of gene expression) is exceptionally high, and drops to a significantly lower level not long after birth. The only other point of the life cycle during which expression is this high is during the mid- to late-life period, during 50–70 years of age. While the increased expression during the prenatal period can be explained by the rapid growth and formation of the brain tissue, the reason behind the surge of late-life expression remains a topic of ongoing research.[37]

There are many genes and proteins that contribute to the formation and development of the CNS, many of which can be found in the aforementioned links. Of particular importance are those that code for BMPs, BMP inhibitors and SHH. When expressed during early development, BMP's are responsible for the differentiation of epidermal cells from the ventral ectoderm. Inhibitors of BMPs, such as NOG and CHRD, promote differentiation of ectoderm cells into prospective neural tissue on the dorsal side. If any of these genes are improperly regulated, then proper formation and differentiation will not occur. BMP also plays a very important role in the patterning that occurs after the formation of the neural tube. Due to the graded response the cells of the neural tube have to BMP and Shh signaling, these pathways are in competition to determine the fate of preneural cells. BMP promotes dorsal differentiation of pre-neural cells into sensory neurons and Shh promotes ventral differentiation into motor neurons. There are many other genes that help to determine neural fate and proper development include, RELN, SOX9, WNT, Notch and Delta coding genes, HOX, and various cadherin coding genes like CDH1 and CDH2.[36]

A great deal of research has been done on the effects of genes and the formation of the brain and the central nervous system. The following wiki links may prove helpful:
Shh and BMP gradient in the neural tube

Development

The study of alcoholism and the neurogenetic factors that increase one's susceptibility is a budding field of study. A multitude of genes associated with the condition have been found which can act as indicators for an individual’s predisposition to alcoholism. Improper expression of ALDH2 and ADH1B leads to polymorphism and causes these two enzymes to function improperly, making it difficult to digest alcohol. This type of expression has been found to be a strong indicator of alcoholism, along with the presence of GABRA2, a gene which codes for a specific GABA receptor. How GABRA2 leads to alcohol dependence is still unclear, but it is thought to interact negatively with alcohol, altering the behavioral effect and resulting in dependency. In general, these genes code for receptor or digestive proteins, and while having these particular genes does indicate a predisposition towards alcoholism, it is not a definitive determining factor. Like all behavioral traits, genes alone do not determine an individual’s personality or behavior, for the influence of the environment is just as important.[27]

Alcohol dependency

[34] much of the current research is being conducted on zebrafish to identify the underlying genetic and morphological aspects that lead to aggression as well as many other behavioral traits.[35] and was later confirmed by Isabelle Seif's mouse experiment,[28] and aggression,MAO A, which first hinted at the possible linkage between MAO-A deficient Dutch family While studies have been conducted on humans, such as Han Brunner's experiment with a [28], and the down regulation of SERT, both contribute to lowering an individual’s level of aggression.5-HT1A, SERT, also have a direct effect on the level of aggression seen in test subjects. The up regulation of a specific 5-HT receptor, 5-HT transporter, which is partially responsible for the degradation of serotonin and thus aggression control. The genes, as well as the proteins themselves, for the 5-HT receptor, as well as the MAO (5-HT) and the varying genes, proteins and enzymes have on aggression is the focus of studies currently. This pathway has been linked to aggression through its influences on early brain development and morphology, as well as directly regulating an individual’s level of impulsive aggression. One enzyme that researchers believe plays a direct role in aggression control is the enzyme serotonin The effect [34].Darwinian fitnessThroughout the animal kingdom, varying styles, types and levels of aggression can be observed leading scientists to believe that there might be a genetic contribution that has conserved this particular behavioral trait. For some species varying levels of aggression have indeed exhibited direct correlation to a higher level of
Outward displays of aggression are seen in most animals
and aggression control. aggressionThere is also research being conducted on how an individual's genes can cause varying levels of

Aggression

Similarly to impulsivity, varying levels of cognition have been linked to many different genes, several of which are related to dopamine genes expression in frontostriatal circuitry. These genes have been seen to play a role in higher cognitive functions such as learning and motivation, possibly by acting on the reward system in the dopamine pathway. It has been shown that these factors, along with many others not related to dopamine, such as CHRM2, are highly heritable. While many executive functions can be learned through experience and environmental factors, individuals with these specific genes, particularly those with high expression levels, were shown to possess higher cognitive function than those without them.[31] One possible explanation for this is that these genes act as high motivational factor, making these individuals more likely to either develop better cognitive function naturally or participate in activities that result in higher cognitive function by means of experience. Much of this motivation may arise from reward based learning. In this type of learning, a particular outcome is more positive than anticipated, resulting in a higher level of dopamine being released in the brain. Dopamine release was for a long time thought to result in a feeling of pleasure, causing an increase in this behavior. However, recent advances in our understanding of reward prediction and learning[32] are leading researchers to view dopamine simply as a reward-error signal, rather than being responsible for inducing the feeling of pleasure. Over time this reward-seeking behavior will increase synaptic plasticity, resulting in an increase in neuronal connections and faster response times.[33]

Higher cognitive function

A 2008 study found a significant correlation between gene expression and brain structure in both model organisms and humans.[30] The levels of expression of dopamine and serotonin in particular have been found to be very influential on brain structure. DAT and DRD4 genes, both of which code for proteins that contribute to the density of the prefrontal gray matter, also have been found to be especially significant. Individuals with ADHD, specifically those with a DRD 4/4 genotype, were found to have smaller prefrontal gray matter volume than those without the 4/4 genotype, indicating that their level of impulse control would be lower than normal. There are many other genes that can contribute to either brain density or its composition, and further studies are being conducted to determine the significance of each.[26]

This suggests that there are specific areas of the brain that play a direct role in the regulation of behavior. This indicates a possible genetic correlation since all human brains have the same general anatomical make up. [26].myelination and levels of grey matter and white In addition, impulsivity levels have been linked to brain density levels, specifically the density of [30].right lateralized neural circuit, differences in impulsivity have been seen to be directly influenced by a PET scans and fMRI is the inclination of an individual to initiate behavior without adequate forethought. An individual with high impulsivity will be more likely to act in ways that are not generally beneficial or are outside the normal range of action one would expect to see. Through the use of such techniques as Impulsivity
Dopamine molecule

Impulse control

[29] Variations in personalities and behavioral traits seen amongst individuals of the same species could be explained by differing levels of expression of these genes and their corresponding proteins.[27] While it is true that variation between species can appear to be pronounced, at their most basic they share many similar behavior traits which are necessary for survival. Such traits include mating, aggression, foraging, social behavior and sleep patterns. This conservation of behavior across species has led biologists to hypothesize that these traits could possibly have similar, if not the same, genetic causes and pathways. Studies conducted on the genomes of a plethora of organisms have revealed that many organisms have

Cross-species gene conservation

Advances in molecular biology techniques and the species-wide genome project have made it possible to map out an individual's entire genome. Whether genetic or environmental factors are primarily responsible for an individual's personality has long been a topic of debate.[26][27] Thanks to the advances being made in the field of neurogenetics, researchers have begun to tackle this question by beginning to map out genes and correlate them to different personality traits.[26] There is little to no evidence to suggest that the presence of a single gene indicates that an individual will express one style of behavior over another; rather, having a specific gene could make one more predisposed to displaying this type of behavior. It is starting to become clear that most genetically influenced behaviors are due to the effects of multiple genes, in addition to other neurological regulating factors like neurotransmitter levels. Aggression, for example, has been linked to at least 16 different genes, many of which have been shown to have different influences on levels of serotonin and dopamine, neurotransmitter density, and other aspects of brain structure and chemistry.[28] Similar findings have been found in studies of impulsivity and alcoholism.[26] Due to fact that many behavioral characteristics have been conserved across species for generations, researchers are able to use animal subjects such as mice and rats, but also fruit flies, worms, and zebrafish,[19][20] to try to determine specific genes that correlate to behavior and attempt to match these with human genes.[29]

Behavioral neurogenetics

Many research facilities seek out volunteers with certain conditions or illnesses to participate in studies. Model organisms, while important, cannot completely model the complexity of the human body, making volunteers a key part to the progression of research. Along with gathering some basic information about medical history and the extent of their symptoms, samples are taken from the participants, including blood, cerebrospinal fluid, and/or muscle tissue. These tissue samples are then genetically sequenced, and the genomes are added to current database collections. The growth of these data bases will eventually allow researchers to better understand the genetic nuances of these conditions and bring therapy treatments closer to reality. Current areas of interest in this field have a wide range, spanning anywhere from the maintenance of circadian rhythms,[24] the progression of neurodegenerative disorders, the persistence of periodic disorders, and the effects of mitochondrial decay on metabolism.[25]

Human research

In addition to examining how genetic mutations affect the actual structure of the brain, researchers in neurogenetics also examine how these mutations affect cognition and behavior. One method of examining this involves purposely engineering model organisms with mutations of certain genes of interest. These animals are then classically conditioned to perform certain types of tasks, such as pulling a lever in order to gain a reward. The speed of their learning, the retention of the learned behavior, and other factors are then compared to the results of healthy organisms to determine what kind of an effect – if any – the mutation has had on these higher processes. The results of this research can help identify genes that may be associated with conditions involving cognitive and learning deficiencies.[23]

Model organisms are an important tool in many areas of research, including the field of neurogenetics. By studying creatures with simpler nervous systems and with smaller genomes, scientists can better understand their biological processes and apply them to more complex organisms, such as humans. Due to their low-maintenance and highly mapped genomes, mice, Drosophila,[19] and C. elegans[20] are very common. Zebrafish[21] and prairie voles[22] have also become more common, especially in the social and behavioral scopes of neurogenetics.

Zebrafish
Drosophila

Animal research

[18][17]

Recombinant DNA

[16] rather than brain slices. Human beings pose a greater challenge for QTL analysis because the genetic population cannot be as carefully controlled as that of an inbred recombinant population, which can result in sources of statistical error.nuclear magnetic resonance imaging (MRI) QTL mapping can also be carried out in humans, though brain morphologies are examined using [15]

This article was sourced from Creative Commons Attribution-ShareAlike License; additional terms may apply. World Heritage Encyclopedia content is assembled from numerous content providers, Open Access Publishing, and in compliance with The Fair Access to Science and Technology Research Act (FASTR), Wikimedia Foundation, Inc., Public Library of Science, The Encyclopedia of Life, Open Book Publishers (OBP), PubMed, U.S. National Library of Medicine, National Center for Biotechnology Information, U.S. National Library of Medicine, National Institutes of Health (NIH), U.S. Department of Health & Human Services, and USA.gov, which sources content from all federal, state, local, tribal, and territorial government publication portals (.gov, .mil, .edu). Funding for USA.gov and content contributors is made possible from the U.S. Congress, E-Government Act of 2002.
 
Crowd sourced content that is contributed to World Heritage Encyclopedia is peer reviewed and edited by our editorial staff to ensure quality scholarly research articles.
 
By using this site, you agree to the Terms of Use and Privacy Policy. World Heritage Encyclopedia™ is a registered trademark of the World Public Library Association, a non-profit organization.
 



Copyright © World Library Foundation. All rights reserved. eBooks from Hawaii eBook Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.