Promoter bashing

Promoter bashing is a technique used in molecular biology to identify the importance of a specific promoter for the proper expression of the gene. Specific point mutations are made and the transcription of the gene is then measured. The importance of a promoter can be observed by the level of transcription.

1. Clone the region of DNA thought to act as a promoter
2. Sequence the region
3. Digest with appropriate restriction enzymes
4. Ligate to reporter gene so that the reporter can be transcribed in the cells of interest
5. Transform cells of interest with the various promoter:reporter constructs
6. Measure reporter-gene transcription rates by assaying the reporter gene product

References

This article was sourced from Creative Commons Attribution-ShareAlike License; additional terms may apply. World Heritage Encyclopedia content is assembled from numerous content providers, Open Access Publishing, and in compliance with The Fair Access to Science and Technology Research Act (FASTR), Wikimedia Foundation, Inc., Public Library of Science, The Encyclopedia of Life, Open Book Publishers (OBP), PubMed, U.S. National Library of Medicine, National Center for Biotechnology Information, U.S. National Library of Medicine, National Institutes of Health (NIH), U.S. Department of Health & Human Services, and USA.gov, which sources content from all federal, state, local, tribal, and territorial government publication portals (.gov, .mil, .edu). Funding for USA.gov and content contributors is made possible from the U.S. Congress, E-Government Act of 2002.
 
Crowd sourced content that is contributed to World Heritage Encyclopedia is peer reviewed and edited by our editorial staff to ensure quality scholarly research articles.
 
By using this site, you agree to the Terms of Use and Privacy Policy. World Heritage Encyclopedia™ is a registered trademark of the World Public Library Association, a non-profit organization.