This article will be permanently flagged as inappropriate and made unaccessible to everyone. Are you certain this article is inappropriate? Excessive Violence Sexual Content Political / Social
Email Address:
Article Id: WHEBN0000102505 Reproduction Date:
The Protein Data Bank (PDB) is a repository for the three-dimensional structural data of large biological molecules, such as Worldwide Protein Data Bank, wwPDB.
The PDB is a key resource in areas of structural biology, such as structural genomics. Most major scientific journals, and some funding agencies, now require scientists to submit their structure data to the PDB. If the contents of the PDB are thought of as primary data, then there are hundreds of derived (i.e., secondary) databases that categorize the data differently. For example, both SCOP and CATH categorize structures according to type of structure and assumed evolutionary relations; GO categorize structures based on genes.[4]
Two forces converged to initiate the PDB: 1) a small but growing collection of sets of protein structure data determined by X-ray diffraction; and 2) the newly available (1968) molecular graphics display, the Brookhaven RAster Display (BRAD), to visualize these protein structures in 3-D. In 1969, with the sponsorship of Walter Hamilton at the Brookhaven National Laboratory, Edgar Meyer (Texas A&M University) began to write software to store atomic coordinate files in a common format to make them available for geometric and graphical evaluation. By 1971, one of Meyer's programs, SEARCH, enabled researchers to remotely access information from the database to study protein structures offline.[5] SEARCH was instrumental in enabling networking, thus marking the functional beginning of the PDB.
Upon Hamilton's death in 1973, Tom Koeztle took over direction of the PDB for the subsequent 20 years. In January 1994,
The structure files may be viewed using one of several open source computer programs. Some other free, but not open source programs include ICM-Browser,[18] VMD, MDL Chime, Pymol, UCSF Chimera, Rasmol, Swiss-PDB Viewer,[19] StarBiochem[20] (a Java-based interactive molecular viewer with integrated search of protein databank), Sirius, and VisProt3DS[21] (a tool for Protein Visualization in 3D stereoscopic view in anaglyth and other modes). The RCSB PDB website contains an extensive list of both free and commercial molecule visualization programs and web browser plugins.
The "4hhb" is the PDB identifier. Each structure published in PDB receives a four-character alphanumeric identifier, its PDB ID. (This cannot be used as an identifier for biomolecules, because often several structures for the same molecule—in different environments or conformations—are contained in PDB with different PDB IDs.)
4hhb
http://www.pdb.org/pdb/files/4hhb.pdb.gz or http://pdbe.org/download/4hhb
http://www.pdb.org/pdb/files/4hhb.xml.gz or http://pdbe.org/pdbml/4hhb
The file format initially used by the PDB was called the PDB file format. This original format was restricted by the width of computer punch cards to 80 characters per line. Around 1996, the "macromolecular Crystallographic Information file" format, mmCIF, started to be phased in. An XML version of this format, called PDBML, was described in 2005.[17] The structure files can be downloaded in any of these three formats. In fact, individual files are easily downloaded into graphics packages using web addresses:
Note: searchable structures vary over time as some become obsolete and are removed from the database.
The significance of the structure factor files, mentioned above, is that, for PDB structures determined by X-ray diffraction that have a structure file, the electron density map may be viewed. The data of such structures is stored on the "electron density server".[13][14]
These data show that most structures are determined by X-ray diffraction, but about 10% of structures are now determined by protein NMR. When using X-ray diffraction, approximations of the coordinates of the atoms of the protein are obtained, whereas estimations of the distances between pairs of atoms of the protein are found through NMR experiments. Therefore, the final conformation of the protein is obtained, in the latter case, by solving a distance geometry problem. A few proteins are determined by cryo-electron microscopy. (Clicking on the numbers in the original table will bring up examples of structures determined by that method.)
for this validation software has been made available to the public at no charge). [11] The data are then automatically checked for plausibility (the source code[10] can act as deposition, data processing and distribution centers for PDB data. The data processing refers to the fact that wwPDB staff review and annotate each submitted entry.wwPDB joined in 2006. Each of the four members of [9] The BMRB[2] RCSB (USA), and PDBj (Japan).[1]
Silicon, Electromagnetic radiation, Carbon, Copper, Oxygen
Deuterium, Protein, Accuracy and precision, Nuclear magnetic resonance, X-ray crystallography
Computer science, Statistics, Phylogenetics, Evolutionary biology, Biology
Pfam, InterPro, Public domain, PDBsum, Protein Data Bank
Pfam, InterPro, Public domain, Merops, PDBsum
Pfam, InterPro, PDBsum, Protein Data Bank, Bacteria
Pfam, InterPro, PDBsum, Public domain, Protein Data Bank